Effect of trivalent metal ions on phase separation and membrane lipid packing: role in lipid peroxidation.
Identifieur interne : 004150 ( Main/Exploration ); précédent : 004149; suivant : 004151Effect of trivalent metal ions on phase separation and membrane lipid packing: role in lipid peroxidation.
Auteurs : RBID : pubmed:9015396English descriptors
- KwdEn :
- Aluminum (toxicity), Animals, Beryllium (toxicity), Cations (chemistry), Cations (toxicity), Electron Spin Resonance Spectroscopy, Gallium (toxicity), Indium (toxicity), Lipid Peroxidation (drug effects), Liposomes, Membrane Fluidity (drug effects), Membrane Lipids (chemistry), Metals (chemistry), Metals (toxicity), Molecular Structure, Scandium (toxicity), Thermodynamics, Yttrium (toxicity).
- MESH :
- chemical , chemistry : Cations, Membrane Lipids, Metals.
- chemical , toxicity : Aluminum, Beryllium, Cations, Gallium, Indium, Metals, Scandium, Yttrium.
- drug effects : Lipid Peroxidation, Membrane Fluidity.
- Animals, Electron Spin Resonance Spectroscopy, Liposomes, Molecular Structure, Thermodynamics.
Abstract
The capacity of Al3+-related cations (Sc3+, Ga3+, In3+, Be2+, Y3+, and La3+) to promote membrane rigidification and lateral phase separation was evaluated in liposomes containing zwitterionic (phosphatidylcholine, PC) and negatively charged (phosphatidylserine, PS) phospholipids. These effects were correlated with the capacity of the ions to stimulate Fe2+-supported lipid peroxidation. A13+, Sc3+, Ga3+, In3+, Be2+, Y3+, and La3+ (50-200 microM) increased the order parameter of the fluorescent probe 1,3-diphenylhexatriene incorporated in PC:PS membranes. In addition, the electron paramagnetic resonance spectra of spin-labeled fatty acids indicated a reduction in lipid motion induced by Sc3+, Y3+, and La3+. The effect was found to extend down to carbon 16 on the acyl chain. The ions (10-200 microM) were also able to induce lateral phase separation, as evaluated from the increase in fluorescence quenching of the probe 2-(6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecanoyl-1-hexadec anoyl-sn-glycero-3-phosphocholine. The ability of the ions to alter membrane lipid packing and induce lateral phase separation correlated in a positive manner (r2 = 0.91 and 0.90, respectively) with their capacity to stimulate the production of Fe2+-initiated 2-thiobarbituric-reactive species, a measure of lipid peroxidation. These results show that Al3+-related metal ions cause membrane rigidification and phase separation, which could affect membrane-related processes. The results support the hypothesis that ions without redox capacity can stimulate Fe2+-initiated lipid peroxidation by increasing lipid packing and by promoting the formation of rigid clusters. Both processes will bring phospholipid acyl chains closer together, thus favoring the propagation step of lipid peroxidation.
DOI: 10.1006/abbi.1996.9810
PubMed: 9015396
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effect of trivalent metal ions on phase separation and membrane lipid packing: role in lipid peroxidation.</title><author><name sortKey="Verstraeten, S V" uniqKey="Verstraeten S">S V Verstraeten</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Quimica Biologica, IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Departamento de Quimica Biologica, IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires</wicri:regionArea></affiliation></author><author><name sortKey="Nogueira, L V" uniqKey="Nogueira L">L V Nogueira</name></author><author><name sortKey="Schreier, S" uniqKey="Schreier S">S Schreier</name></author><author><name sortKey="Oteiza, P I" uniqKey="Oteiza P">P I Oteiza</name></author></titleStmt><publicationStmt><date when="1997">1997</date><idno type="RBID">pubmed:9015396</idno><idno type="pmid">9015396</idno><idno type="doi">10.1006/abbi.1996.9810</idno><idno type="wicri:Area/Main/Corpus">004434</idno><idno type="wicri:Area/Main/Curation">004434</idno><idno type="wicri:Area/Main/Exploration">004150</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aluminum (toxicity)</term><term>Animals</term><term>Beryllium (toxicity)</term><term>Cations (chemistry)</term><term>Cations (toxicity)</term><term>Electron Spin Resonance Spectroscopy</term><term>Gallium (toxicity)</term><term>Indium (toxicity)</term><term>Lipid Peroxidation (drug effects)</term><term>Liposomes</term><term>Membrane Fluidity (drug effects)</term><term>Membrane Lipids (chemistry)</term><term>Metals (chemistry)</term><term>Metals (toxicity)</term><term>Molecular Structure</term><term>Scandium (toxicity)</term><term>Thermodynamics</term><term>Yttrium (toxicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cations</term><term>Membrane Lipids</term><term>Metals</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Aluminum</term><term>Beryllium</term><term>Cations</term><term>Gallium</term><term>Indium</term><term>Metals</term><term>Scandium</term><term>Yttrium</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lipid Peroxidation</term><term>Membrane Fluidity</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Electron Spin Resonance Spectroscopy</term><term>Liposomes</term><term>Molecular Structure</term><term>Thermodynamics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The capacity of Al3+-related cations (Sc3+, Ga3+, In3+, Be2+, Y3+, and La3+) to promote membrane rigidification and lateral phase separation was evaluated in liposomes containing zwitterionic (phosphatidylcholine, PC) and negatively charged (phosphatidylserine, PS) phospholipids. These effects were correlated with the capacity of the ions to stimulate Fe2+-supported lipid peroxidation. A13+, Sc3+, Ga3+, In3+, Be2+, Y3+, and La3+ (50-200 microM) increased the order parameter of the fluorescent probe 1,3-diphenylhexatriene incorporated in PC:PS membranes. In addition, the electron paramagnetic resonance spectra of spin-labeled fatty acids indicated a reduction in lipid motion induced by Sc3+, Y3+, and La3+. The effect was found to extend down to carbon 16 on the acyl chain. The ions (10-200 microM) were also able to induce lateral phase separation, as evaluated from the increase in fluorescence quenching of the probe 2-(6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecanoyl-1-hexadec anoyl-sn-glycero-3-phosphocholine. The ability of the ions to alter membrane lipid packing and induce lateral phase separation correlated in a positive manner (r2 = 0.91 and 0.90, respectively) with their capacity to stimulate the production of Fe2+-initiated 2-thiobarbituric-reactive species, a measure of lipid peroxidation. These results show that Al3+-related metal ions cause membrane rigidification and phase separation, which could affect membrane-related processes. The results support the hypothesis that ions without redox capacity can stimulate Fe2+-initiated lipid peroxidation by increasing lipid packing and by promoting the formation of rigid clusters. Both processes will bring phospholipid acyl chains closer together, thus favoring the propagation step of lipid peroxidation.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">9015396</PMID><DateCreated><Year>1997</Year><Month>02</Month><Day>24</Day></DateCreated><DateCompleted><Year>1997</Year><Month>02</Month><Day>24</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0003-9861</ISSN><JournalIssue CitedMedium="Print"><Volume>338</Volume><Issue>1</Issue><PubDate><Year>1997</Year><Month>Feb</Month><Day>1</Day></PubDate></JournalIssue><Title>Archives of biochemistry and biophysics</Title><ISOAbbreviation>Arch. Biochem. Biophys.</ISOAbbreviation></Journal><ArticleTitle>Effect of trivalent metal ions on phase separation and membrane lipid packing: role in lipid peroxidation.</ArticleTitle><Pagination><MedlinePgn>121-7</MedlinePgn></Pagination><Abstract><AbstractText>The capacity of Al3+-related cations (Sc3+, Ga3+, In3+, Be2+, Y3+, and La3+) to promote membrane rigidification and lateral phase separation was evaluated in liposomes containing zwitterionic (phosphatidylcholine, PC) and negatively charged (phosphatidylserine, PS) phospholipids. These effects were correlated with the capacity of the ions to stimulate Fe2+-supported lipid peroxidation. A13+, Sc3+, Ga3+, In3+, Be2+, Y3+, and La3+ (50-200 microM) increased the order parameter of the fluorescent probe 1,3-diphenylhexatriene incorporated in PC:PS membranes. In addition, the electron paramagnetic resonance spectra of spin-labeled fatty acids indicated a reduction in lipid motion induced by Sc3+, Y3+, and La3+. The effect was found to extend down to carbon 16 on the acyl chain. The ions (10-200 microM) were also able to induce lateral phase separation, as evaluated from the increase in fluorescence quenching of the probe 2-(6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecanoyl-1-hexadec anoyl-sn-glycero-3-phosphocholine. The ability of the ions to alter membrane lipid packing and induce lateral phase separation correlated in a positive manner (r2 = 0.91 and 0.90, respectively) with their capacity to stimulate the production of Fe2+-initiated 2-thiobarbituric-reactive species, a measure of lipid peroxidation. These results show that Al3+-related metal ions cause membrane rigidification and phase separation, which could affect membrane-related processes. The results support the hypothesis that ions without redox capacity can stimulate Fe2+-initiated lipid peroxidation by increasing lipid packing and by promoting the formation of rigid clusters. Both processes will bring phospholipid acyl chains closer together, thus favoring the propagation step of lipid peroxidation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Verstraeten</LastName><ForeName>S V</ForeName><Initials>SV</Initials><Affiliation>Departamento de Quimica Biologica, IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.</Affiliation></Author><Author ValidYN="Y"><LastName>Nogueira</LastName><ForeName>L V</ForeName><Initials>LV</Initials></Author><Author ValidYN="Y"><LastName>Schreier</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Oteiza</LastName><ForeName>P I</ForeName><Initials>PI</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>In Vitro</PublicationType><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>UNITED STATES</Country><MedlineTA>Arch Biochem Biophys</MedlineTA><NlmUniqueID>0372430</NlmUniqueID><ISSNLinking>0003-9861</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Cations</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Liposomes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Membrane Lipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Metals</NameOfSubstance></Chemical><Chemical><RegistryNumber>045A6V3VFX</RegistryNumber><NameOfSubstance>Indium</NameOfSubstance></Chemical><Chemical><RegistryNumber>58784XQC3Y</RegistryNumber><NameOfSubstance>Yttrium</NameOfSubstance></Chemical><Chemical><RegistryNumber>CH46OC8YV4</RegistryNumber><NameOfSubstance>Gallium</NameOfSubstance></Chemical><Chemical><RegistryNumber>CPD4NFA903</RegistryNumber><NameOfSubstance>Aluminum</NameOfSubstance></Chemical><Chemical><RegistryNumber>OW5102UV6N</RegistryNumber><NameOfSubstance>Beryllium</NameOfSubstance></Chemical><Chemical><RegistryNumber>YUJ4U1EW7R</RegistryNumber><NameOfSubstance>Scandium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Aluminum</DescriptorName><QualifierName MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Beryllium</DescriptorName><QualifierName MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cations</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName><QualifierName MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electron Spin Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Gallium</DescriptorName><QualifierName MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium</DescriptorName><QualifierName MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Lipid Peroxidation</DescriptorName><QualifierName MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Liposomes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Membrane Fluidity</DescriptorName><QualifierName MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Membrane Lipids</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Metals</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName><QualifierName MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Scandium</DescriptorName><QualifierName MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Thermodynamics</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Yttrium</DescriptorName><QualifierName MajorTopicYN="N">toxicity</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>2</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9015396</ArticleId><ArticleId IdType="pii">S0003-9861(96)99810-0</ArticleId><ArticleId IdType="doi">10.1006/abbi.1996.9810</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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